Distribution and antibiotic resistance of the clinical bacterial isolated from the ICU of Tongren Hospital during 2013-2017

Abstract

Objective To analyze the distribution and antibiotic resistance of the clinical bacterial isolated from the intensive care unit (ICU) of Tongren Hospital during 2013-2017. Methods All the bacterial isolates were identified by Vitek-2 Compact system. Antimicrobial susceptibility testing was carried out using Kirby-Bauer method or automated systems. The susceptibility data were analyzed using WHONET 5.6 software. Results were interpreted according to CLSI M100-S27th breakpoints. Results A total of 1 049 clinical isolates were collected, including 331 (31.6%) strains of gram positive bacteria and 718 (68.4%) strains of gram negative bacteria. The overall prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Staphylococcus aureus and methicillin resistant coagulase negative staphylococci (MRCNS) in coagulase-negative Staphylococcus were 84.5% and 80.2%. No Staphylococcus strains were found resistant to vancomycin or linezolid. The resistance rates of E. faecalis strains to most drugs tested were lower than those of E. faecium. Polymerase chain reaction (PCR) amplification and sequencing analysis indicated that the only one vancomycin-resistant strains isolated from E. faecium was vanM type. The resistance rates to carbapenems of Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii respectively were 50.7%, 50.4%, and 82.7%. Conclusions Antibiotic resistance is still very serious in ICU. It is necessary to maintain good practice in surveillance of bacterial resistance, and strengthen management of clinical use of antimicrobial agents and hospital infection control. Key words: Intensive care units; Microbial sensitivity tests; Drug resistance, bacterial