Abstract
The distribution of active compounds of traditional Chinese medicine Salvia miltiorrhiza Bunge (Chinese name: Danshen) in vivo was determined by establishing a liquid extraction surface analysis coupled with the tandem mass spectrometry (LESA-MS/MS) method. Stability analysis and distribution analysis were designed in the present study using normal animals or a myocardial ischemia model. The model assessment was performed four weeks after surgery, and then three groups were created: a normal-dose group, a model-blank group, and a model-dose group. Meanwhile, Danshen decoction administration began in dose groups and lasted for four weeks. In stability analysis, four salvianolic acids—Danshensu (DSS), caffeic acid (CAA), rosmarinic acid (RA), and salvianolic acid A (SAA)—in kidney tissues from the normal-dose group were detected by LESA-MS/MS under four conditions, and then distribution analysis was conducted in different tissues using the same method. Ejection fraction (EF) and fractional shortening (FS) in animals from two model groups decreased significantly four weeks after surgery (P < 0.01) and were improved after four weeks of Danshen decoction administration (P < 0.01). Results of stability analysis demonstrated that this method was basically stable since there were no significant differences in signal intensities of DSS, CAA, and SAA under four conditions (P > 0.05). Distribution analysis showed the signal intensities of DSS in the liver and kidney and SAA in the heart were higher in the model-dose group than in the normal-dose group (P < 0.05 or P < 0.01). Signal intensities of RA in the liver and kidney, and SAA in the liver were lower in the model-dose group compared with the normal-dose group (P < 0.05 or P < 0.01). In conclusion, Danshen decoction has the effect of improving the ischemic condition in a chronic myocardial ischemia model, and the content of two active compounds increased in the targets. These findings contribute to an understanding of the therapeutic role of Danshen in cardiovascular disease.
Highlights
Cardiovascular disease (CVD), which is defined as a set of diseases and conditions including coronary heart disease (CHD), cerebrovascular disease, and heart failure, has been the leading cause of mortality across the world [1]
Echocardiography showed that the Ejection fraction (EF) of six animals was less than 60%, which supported the diagnosis of myocardial ischemia
After treated with Danshen decoction for four weeks, EF and fractional shortening (FS) of pigs in the model-dose group were improved significantly than before (P < 0.05) and had obvious differences when compared with the model-blank group (P < 0.01) (Figure 3)
Summary
Cardiovascular disease (CVD), which is defined as a set of diseases and conditions including coronary heart disease (CHD), cerebrovascular disease, and heart failure, has been the leading cause of mortality across the world [1]. CHD, as the most important disease of CVD, has climbed from the seventh leading cause of death in China in 1990 to the second today [2]. In China, TCM as a complementary therapy has been widely used for CVD. E TCM-Danshen is the dry root and rhizome of Salvia miltiorrhiza Bunge and was first recorded in Shennong Herbal Classic. It is commonly used in the treatment of cardiovascular system, digestive system, and nervous system diseases. E theory of TCM considers Danshen an important medicine for expanding blood vessels, promoting blood circulation, eliminating blood stasis, and relieving pain [3]. It is commonly used in the treatment of cardiovascular system, digestive system, and nervous system diseases. e theory of TCM considers Danshen an important medicine for expanding blood vessels, promoting blood circulation, eliminating blood stasis, and relieving pain [3]. e main chemical
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Evidence-Based Complementary and Alternative Medicine
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
