Abstract
Electron microscopic examination of retinas treated with osmium tetroxide and tris(hydroxymethyl)aminomethane (Tris) showed a disappearance of the lamellae of rod outer segments. The disk edges, surface membrane, cilium and mitochondria of the rod cell remained intact and in their normal relationships. In contrast to rods, the lamellae of cones were unaffected. Treatment of rods with Tris at pH 7.2–7.5, after fixation in phosphate-buffered OsO4, resulted in a loss of brown stain and of refractility of the rods (viewed in the light microscope), which was correlated with the disappearance of the lamellae. The brown soluble material derived from the rod lamellae was fractionated by gel filtration into low- and high-molecular weight components. The proposed mechanism of solubilization of the lamellae is by OsO4 adding to sites of carbon-carbon double bonds, combination of Tris with the addition product then yielding strongly hydrophilic material.
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