Distinctions between cellulase isoenzyme patterns of five plant-pathogenic Verticillium species

Abstract

Cellulase isoenzymes produced in liquid shake cultures by isolates of five species of Verticillium were separated by isoelectric focusing. Each species gave a distinctive pattern (or patterns) of isoenzymes which could be used for identification purposes. There were no differences between the isoenzyme patterns of isolates of the same fungal species even from different host species or showing different degrees of virulence. Filtrates from cultures of different ages did not show any qualitative changes in isoenzyme patterns. The same species-specific patterns of cellulase isoenzymes were produced in media containing either a simple carbon source (carboxymethyl cellulose) or a more complex one (solvent extracted plant tissues).