Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.

Anestis Tsakiridis,Guillaume Blin,Rodrigo Osorno,Yali Huang,Suling Zhao,Valerie Wilson,Eleni Karagianni,Filip Wymeersch,Sally Lowell,Costas Economou,Stavroula Skylaki

doi:10.1242/dev.122093

Abstract

There was an error published in Development 141, 1209-1221.In Fig. 6, NM precursors were wrongly depicted as T(Bra)+Foxa2+ instead of T(Bra)+Sox2+. The corrected figure appears below. The authors apologise to readers for this mistake.

Highlights

Lineage specification in the mouse embryo during gastrulation generates progressively more restricted precursors from initially uncommitted postimplantation epiblast cells (Tzouanacou et al, 2009)
Immunocytochemistry revealed that the early primitive streak (PS) marker T(Bra) (Rivera-Perez and Magnuson, 2005) is expressed heterogeneously in undifferentiated epiblast stem cells (EpiSCs) irrespective of whether they were derived from embryonic stem cells (ESCs) in vitro or from the postimplantation epiblast (Fig. 1A; supplementary material Fig. S1)
Acquisition of lineage identity within primed pluripotency Heterogeneity is prevalent in mouse and human pluripotent cell populations (Chambers et al, 2007; Canham et al, 2010; Blauwkamp et al, 2012; Davies et al, 2013) but few studies have focused on EpiSCs, and these have defined only minor (0.5-1.5%) functionally distinct subpopulations (Hayashi and Surani, 2009; Han et al, 2010)

Summary

Introduction

Lineage specification in the mouse embryo during gastrulation generates progressively more restricted precursors from initially uncommitted postimplantation epiblast cells (Tzouanacou et al, 2009). Pluripotency is widespread in the epiblast until around the start of MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK. Clonal analysis showed that some early-ingressing mesodermal derivatives arise from a common mesendodermal (ME) precursor, whereas lateringressing somitic mesoderm is generated by a neuromesodermal (NM) progenitor, which gives rise to the neurectoderm of the spinal cord (Tzouanacou et al, 2009)

Methods

Results

Conclusion