Abstract
Abstract Objective: To evaluate the role of IL-10 in regulation of peanut-driven cytokine production in peanut allergic, sensitized, and clinically tolerant human populations. Methods: Eighteen clinically peanut allergic, 8 sensitized, and 29 peanut non-allergic individuals between 6-45y were studied. PBMC were stimulated with peanut Ag alone, and in the presence of rIL-10 or anti-IL-10R neutralising Ab in short-term primary cultures. Peanut-driven Type 1, Type 2, and regulatory cytokine response profiles were quantified by ELISA. Results: IL-10 production in response to peanut-specific stimulation was higher in peanut allergic than non-allergic individuals. Exogenous rIL-10 abrogates peanut-driven cytokine production in peanut allergics. Blocking endogenous IL-10 function has no impact on recall Type 2 cytokine responses in non-allergics. Among peanut allergics, anti-IL-10R treatment during Ag stimulation enhanced both Th2 and Th1 recall responses. Conclusion: IL-10 and the IL-10R play no detectable role in preventing initiation of peanut specific food allergy, but may be important in limiting the intensity of peanut specific Th2 and pro-inflammatory responses in peanut allergic individuals.
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