Distinct roles of CpcG1-phycobilisome and CpcG2-phycobilisome in state transitions in a cyanobacterium Synechocystis sp. PCC 6803

Abstract

State transitions in cyanobacteria regulate the relative energy transfer from phycobilisome to photosystem I and II. Although it has been shown that phycobilisome mobility is essential for phycobilisome-dependent state transitions, the biochemical mechanism is not known. Previously we reported that two distinct forms of phycobilisome are assembled with different CpcG copies, which have been referred to as "rod-core linker," in a cyanobacterium Synechocystis sp. PCC 6803. CpcG2-phycobilisome is devoid of a typical central core, while CpcG1-phycobilisome is equivalent to the conventional phycobilisome supercomplex. Here, we demonstrated that the cpcG1 disruptant has a severe specific defect in the phycobilisome-dependent state transition. However, fluorescence recovery after photobleaching measurements showed no obvious difference in phycobilisome mobility between the wild type and the cpcG1 disruptant. This suggests that both CpcG1 and CpcG2 phycobilisomes have an unstable interaction with the reaction centres. However, only CpcG1 phycobilisomes are involved in state transitions. This suggests that state transitions require the phycobilisome core.