Distinct roles for strigolactones in cyst nematode parasitism of Arabidopsis roots

Carmen M Escudero Martinez,Carolien Ruyter-Spira,Casper Van Schaik,Nina Guarneri,Harro Bouwmeester,Hein Overmars,Aska Goverse

doi:10.1007/s10658-019-01691-5

Abstract

Phytohormones play an essential role in different stages of plant-nematode interactions. Strigolactones (SLs) are a novel class of plant hormones which play an important role in plant development. Furthermore, certain soil-inhabiting organisms exploit this plant molecule as allelochemical. However, whether SLs play a role in plant parasitism by nematodes is as yet unknown. This prompted us to investigate the potential role of SLs in different stages of the nematode life cycle using the beet cyst nematode Heterodera schachtii and Arabidopsis as a model system. We analyzed the effect of SLs on cyst nematode hatching, host attraction and invasion, and the establishment of a feeding relation upon infection of the SL deficient mutant max4-1 and the SL signaling mutant max2-1. In addition, infection assays were performed under phosphate shortage to enhance SL production and in the presence of the synthetic SL analog GR24. From this study, we can conclude that SLs do not contribute to cyst nematode hatching at the levels tested but that they do play a role in host attraction and subsequent invasion in a MAX2 dependent manner. Furthermore, we observed that increased levels of exogenous and endogenous SLs change the root invasion zone. Upon root infection, cyst nematode development was enhanced in both the max2-1 and max4-1 mutants due to the formation of enlarged feeding cells. These data provide evidence for distinct roles of SLs during cyst nematode parasitism of plant roots.

Highlights

Cyst nematodes (CN) of the genera Heterodera and Globodera belong to the most harmful plant-parasitic nematodes for a number of major food crops (Jones et al 2013)
We tested if SLs could potentially act as hatching factor for H. schachtii by evaluating the hatching activity of the synthetic SL analogue GR24
Hatching of pre-parasitic stage juveniles (J2) from eggs is enhanced by the release of rootdiffusates of these host plants, but not exclusively, as some juveniles spontaneously hatch in water (Clarke and Shepherd 1964)

Summary

Introduction

Cyst nematodes (CN) of the genera Heterodera and Globodera belong to the most harmful plant-parasitic nematodes for a number of major food crops (Jones et al 2013). Worldwide annual crop losses caused by plant-parasitic nematodes have been estimated to exceed $157 billion U.S dollars (Abad et al 2008). In high densities, these nematodes disrupt the physiology of their host plant, decreasing their fitness and causing reductions in crop yield and quality. Specific symptoms are the cutinized cysts found either along the root surface or in the soil. They remain in the soil for decades and make the control of cyst nematodes difficult and complex (Jones et al 2013)

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Conclusion