Abstract
The small proline-rich protein, SPRR1, is a marker gene whose expression in conducting airway epithelium is elevated under a variety of conditions that enhance squamous differentiation. The purpose of this study is to elucidate the nature of the SPRR1 sequence involved in cross-linked envelope formation in a tissue/cell type, such as conducting airway epithelium, that normally does not express squamous function except after injury or maintenance in culture. For this, a Flag-SPRR1 fusion protein expression system has been developed. Using the liposome-mediated gene transfer technique on passage 1 culture of human tracheobronchial epithelial (TBE) cells, the Flag-SPRR1 fusion protein can be expressed and detected immunologically by both anti-Flag and anti-SPRR1 antibodies. The incorporation of Flag-SPRR1 fusion protein into cross-linked envelopes can be demonstrated when transfected human passage 1 TBE cultures are treated with phorbol 12-myristate 13-acetate and high calcium (1.5 mM). By deletion and site-directed mutagenesis, two distinct roles of the amino- and carboxyl-terminal sequences of SPRR1 have been demonstrated. First, we demonstrated that the amino-terminal sequence of SPRR1 protein is required for the incorporation of the fusion protein into cross-linked envelopes, whereas a deletion on the carboxyl-terminal region or on the middle repetitive unit has no effect. Interestingly, insertion of a 24-amino acid peptide of monkey MUC2 repetitive sequence in the amino-terminus of SPRR1 protein had a stimulatory effect. Site-directed mutagenesis on the following amino acid residues, Lys(7), Gln(88), and Lys(89), which were found previously to participate in the cross-linked envelope formation of keratinocytes, had no detrimental effect on the incorporation. However, mutations on Gln clusters, such as Gln(4)-Gln(6) and Gln(22)-Gln(25), had detrimental effects on the incorporation. These results suggest an amino-terminal sequence-dependent and multiple cross-linked sites for the incorporation of Flag-SPRR1 fusion protein into cross-linked envelopes of cultured human TBE cells. Second, we demonstrated that the carboxyl terminus of SPRR1 protein is required for a high level of Flag-fusion protein expression. A deletion in the carboxyl region or a mutation on the last lysine residue of the carboxyl end had a detrimental effect on the level of Flag-SPRR1 fusion protein expressed in transfected cells. In contrast, there was only a slight decrease in the level of expression if the amino-terminus was deleted. Interestingly, the efficiency for fusion protein to incorporate into cross-linked envelopes was elevated by the mutation at the carboxyl end. These results suggest distinct roles, perhaps coordinately, for both amino- and carboxyl-terminal sequences in the regulation of the life cycle of SPRR1 protein in cultured TBE cells.
Highlights
The carboxyl end had a detrimental effect on the level of Flag-SPRR1 fusion protein expressed in transfected cells
SPRR1 protein structure is characterized by the presence of internal repeats and conserved amino acid sequences of both amino- and carboxyl-terminal regions across species [11], including proteins isolated from monkey tracheobronchial epithelial (TBE) cells [12]
Characterization of Flag-SPRR1 Fusion Protein Expression in Transfected TBE Cells—To verify whether the pFlag-SPRR1 construct expresses the correct fusion protein, the synthesis of the fusion protein was characterized in BEAS-2B (S) cell line by transfection
Summary
The carboxyl end had a detrimental effect on the level of Flag-SPRR1 fusion protein expressed in transfected cells. The efficiency for fusion protein to incorporate into cross-linked envelopes was elevated by the mutation at the carboxyl end These results suggest distinct roles, perhaps coordinately, for both amino- and carboxyl-terminal sequences in the regulation of the life cycle of SPRR1 protein in cultured TBE cells. Immunohistochemical studies, using a polyclonal antibody against carboxyl-terminal peptide (15 amino acid) of the human/monkey SPRR1 protein, demonstrated that SPRR1 is predominantly present in the suprabasal cell layer of various squamous tissues such as epidermis, oral mucosa, and esophagus [10].2. This tissue- and cell-type-specific distribution of SPRR1 gene expression suggests a useful marker associated with squamous differentiation. The functional role of SPRR1 in the terminal squamous differentiation of TBE cells remains unknown
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