Abstract
Long interspersed nuclear element (LINE) is known to be transposed by the reverse transcription using its RNA transcript. Recognition of the 3' stem-loop of LINE RNA by its reverse transcriptase (RT) is an important step of the retrotransposition. We focused on the RNA recognition by RT from two related LINEs, ZfL2-1 and ZfL2-2, from zebrafish. Previous study showed that RT from ZfL2-2 recognizes a single residue in the specific position of the RNA loop. In the present study, it was found that RT from ZfL2-1 recognizes the inserted stem-loop of ZfL2-1 RNA. Thus, these related RTs recognize the same region of LINE RNAs but discriminate them by different mechanism.
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