Distinct genotoxicity of phenylmercury acetate in human lymphocytes as compared with other mercury compounds

Abstract

In the present study, the frequency of sister chromatid exchanges (SCEs) was assayed to evaluate the genotoxic effects of mercury nitrate (Hg 2+), methylmercury chloride (CH 3HgCl) and phenylmercury acetate (PMA) on human lymphocytes. The free radical scavengers, catalase (CA) and superoxide dismutase (SOD) were tested for their antigenotoxic effects toward PMA. PMA (1–30 μM) increased SCE frequency in a concentration-dependent manner. However, CH 3HgCl significantly increased SCE frequency only at a concentration of 20 μM, and all concentrations treated with Hg 2+ did not induce a positive effect. On the other hand, we first reported that 30 μM Hg 2+, 20 μM CH 3HgCl and (3–30 μM) PMA significantly increased the frequency of endoreduplicated mitosis. PMA was about 3- or 5-fold more effective in inducing endoreduplication than CH 3HgCl or Hg 2+ at equivalent toxic concentrations, respectively. However, neither CA nor SOD in concentrations of 75 and 150 μg/ml showed antagonistic action on the genotoxic effects of PMA. The results suggest that the mechanism of PMA-induced genotoxicity is not mediated by superoxide anion nor H 2O 2. It is concluded that PMA, which was more effective in inducing the elevation of both SCEs and endoreduplication, may be especially hazardous of the three mercury compounds tested.