Abstract
This study sought to identify potential bioactive peptides from the placenta that are involved in preeclampsia (PE) to obtain information about the prediction, diagnosis and treatment of PE. The liquid chromatography/mass spectrometry was used to perform a comparative analysis of placental peptides in normal and PE pregnancies. Gene ontology (GO), pathway analysis and ingenuity pathway analysis (IPA) were used to evaluate the underlying biological function of the differential peptides based on their protein precursors. Transwell assays and qPCR were used to study the effect of the identified bioactive peptides on the function of HTR-8/SVneo cells. A total of 392 upregulated peptides and 420 downregulated peptides were identified (absolute fold change ≥ 2 and adjusted P value < 0.05). The GO analysis, pathway analysis, and IPA revealed that these differentially expressed peptides play a role in PE. In addition, the up-regulated peptide “DQSATALHFLGRVANPLSTA” derived from Angiotensinogen exhibited effect on the invasiveness of HTR-8/SVneo cells. The current preliminary research not only provides a new research direction for studying the pathogenesis of PE, but also brings new insights for the prediction, diagnosis and treatment of PE.
Highlights
This study sought to identify potential bioactive peptides from the placenta that are involved in preeclampsia (PE) to obtain information about the prediction, diagnosis and treatment of PE
Abbreviations ACOG American College of Obstetricians and Gynecologists BH procedure Benjamini–Hochberg procedure body mass index (BMI) Body mass index fetal bovine serum (FBS) Fetal bovine serum False Discovery Rate (FDR) False discovery rate GO Gene ontology ingenuity pathway analysis (IPA) Ingenuity pathway analysis LC–MS/MS Liquid chromatography–tandem mass spectrometry MMP Matrix metalloproteinases molecular weight (MW) Molecular weight PE Preeclampsia pI Isoelectric point quantitative PCR (qPCR) Quantitative PCR tandem mass tag (TMT) Tandem mass tag Preeclampsia (PE) is a pregnancy complication characterized by abrupt hypertension and signs of maternal dysfunction after 20 weeks of gestation, and is the leading cause of maternal and perinatal morbidity and m ortality[1,2]
We further found that an up-regulated peptide "DQSATALHFLGRVANPLSTA" derived from Angiotensinogen (AGT) cold increase the trophoblast cells invasion though transwell assay
Summary
This study sought to identify potential bioactive peptides from the placenta that are involved in preeclampsia (PE) to obtain information about the prediction, diagnosis and treatment of PE. The liquid chromatography/mass spectrometry was used to perform a comparative analysis of placental peptides in normal and PE pregnancies. Transwell assays and qPCR were used to study the effect of the identified bioactive peptides on the function of HTR-8/SVneo cells. An in-depth analysis is worthwhile to identify differentially expressed placental peptides that are conducive to the diagnosis and treatment of PE, which perhaps may help clinicians predict, diagnose, and treat PE. We aimed to identify potential bioactive peptides involved in PE and help develop promising regimens for prediction, diagnosis and treatment of PE
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