Abstract
MRF4 is a muscle-specific transcription factor that is expressed both in embryonic somites and later in fetal and adult muscle fibers.Cis-regulatory elements of theMRF4gene responsible for its complex expression pattern have not yet been identified, although previous studies of the ratMRF4gene have demonstrated the presence of enhancer activity located several kilobases 5′ to the transcription start site. Using cell transfection assays in vitro, we have now localized one of the regulatory regions ofMRF4to a 590-base-pair sequence between 4 and 5 kilobases upstream from the start site. This sequence region functioned as an enhancer in combination either with theMRF4promoter or with the viralthymidine kinase (tk)promoter. Deletion analysis ofMRF4indicated the existence of another regulatory region, closer to the promoter, which functioned as an enhancer in combination with theMRF4promoter but not with thetkpromoter.
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