Abstract
The DO-stat fed-batch fermentation was carried out to explore the volumetric productivity of β-carotene in engineered Yarrowia lipolytica C11 strain. Using DO-stat fed-batch fermentation, we achieved 94 g/L biomass and 2.01 g/L β-carotene. Both biomass and β-carotene were about 1.28-fold higher than that in fed-batch fermentation. The ATP, NADP+/NADPH, and gene expression levels of tHMG, GGS1, carRA, and carB were promoted as compared to that in fed-batch fermentation. As for as the kinetic parameters in DO-stat fed-batch fermentation, μm′, Yx/s′, and Yp/s′ was 0.527, 0.353, and 0.158, respectively. The μm′ was elevated 4.66-fold than that in fed-batch fermentation. These data illustrate that more dissolved oxygen increased the biomass. The Yx/s′ and Yp/s′ were increased 1.15 and 22.57-fold, which suggest that the DO-stat fed-batch fermentation reduced the Crabtree effect and improved the utilization rate of glucose. Therefore, DO-stat fed-batch fermentation is a promising strategy in the industrialized production of β-carotene.
Highlights
The DO-stat fed-batch fermentation was carried out to explore the volumetric productivity of β-carotene in engineered Yarrowia lipolytica C11 strain
Compared with the non-oleaginous Saccharomyces cerevisiae or Escherichia coli, Y. lipolytica is more suitable for the production of β-carotene
The results demonstrate that more glucose was utilized in DO-stat fed-batch fermentation, and more substrate yield for biomass and product was obtained
Summary
The DO-stat fed-batch fermentation was carried out to explore the volumetric productivity of β-carotene in engineered Yarrowia lipolytica C11 strain. The Yx/s′ and Yp/s′ were increased 1.15 and 22.57-fold, which suggest that the DO-stat fed-batch fermentation reduced the Crabtree effect and improved the utilization rate of glucose. DO-stat fed-batch fermentation is a promising strategy in the industrialized production of β-carotene. The DO-stat fed-batch fermentation strategy was used to produce tyrosine phenol lyase by recombinant Escherichia coli, the final biomass was 35.6 g/L, and the volumetric activity reached 12,292 U/L after 30 h cultivation[34]. The DO-stat feeding strategy was promising together with the use of ammonium hydroxide for pH control to improve P(3HB) volumetric p roductivity[35] This particular method has been widely used in the aerobic culture to produce highly valuable chemicals and biofuels
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