Abstract
The molecular changes in complex dissolved organic matter and the microbial community structure in a laboratory-scale two-phase multi-staged up-flow anaerobic sludge blanket (MS-UASB) system were determined by unknown screening analysis with Orbitrap mass spectrometry and next-generation sequencing using the Illumina® Miseq platform. The acidification (AC) reactor selectively decomposed less oxidized molecules and produced more oxidized compounds with less alkylated groups (lower number of CH2 groups). Subsequently, the MS-UASB removed a wide range of CHO moieties and effectively biodegraded the compounds produced by the AC reactor. However, longer CH2-chain compounds were observed. The retained sludge obtained from the MS-UASB reactor under different operating conditions revealed the dominance of the genus Methanosaeta (an acetoclastic methanogen) in the archaeal community. In addition, the distribution of the microbial community structures of the retained sludge and floating sludge differed when the MS-UASB reactor was unstable.
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