Dissolved organic matter characteristics in soils of tropical legume and non-legume tree plantations

Abstract

Dissolved organic matter (DOM) drives many fundamental biogeochemical processes (e.g., carbon storage, nutrient cycling, and soil development) in forest soil. However, the molecular-level characteristics of DOM derived from different types of tropical forest soils are poorly understood. Here, water samples at different soil depths (0, 20, and 40 cm) from tropical legume (Acacia auriculiformis, AA) and non-legume (Eucalyptus urophylla, EU) tree plantations were analyzed using absorption and fluorescence spectroscopy, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), and solution-state 1H nuclear magnetic resonance (NMR) spectroscopy. The FT-ICR MS results indicated that DOM persisted in the soil, but its molecular composition notably shifted from low-mass (150–300 Da) and more-aromatic molecules to middle- (300–450 Da) and high-mass (>450 Da) and less-aromatic molecules with increasing soil depth. This was primarily mediated by consumption and mineral sorption of low-mass plant-derived DOM (e.g., low-mass carbohydrates and polyphenols) and further formation of larger microbial products (e.g., protein-like and lipid-like compounds). In addition, a higher abundance of microbial-derived molecules (e.g., protein-like and carboxyl-rich alicyclic molecules) was found at the legume plantation relative to the non-legume plantation, which suggests a faster microbial turnover of DOM. Also, the legume plantation had greater enrichment of middle- and high-mass and condensed aromatic-like DOM components in soils. These findings improve our understanding of the drivers that mediate the response of DOM to soil depth and tree species in tropical plantations.