Dissipation of Hexythiozox on Beans Pods by HPLC–DAD

Abstract

An effective analytical method for the residue analysis of a novel acaricide hexythiozox and its dissipation in beans pods were studied. Hexythiozox residues were extracted from beans pods samples and the extract was cleaned up according to QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method and determined by high-performance liquid chromatography with photodiode array detector (HPLC-DAD). At fortification levels of 0.1, 0.5, and 1.0 mg kg(-1) in Beans Pods, it was shown that recoveries ranged from 82.4 % to 89.6 % with relative standard deviation (RSD) of 6 %-9 %. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.02 and 0.06 mg kg(-1), respectively. The dissipation half-life time of hexythiozox residues in beans pods was 12.04 days. According to maximum residue limit (MRL) 0.5 mg kg(-1), the preharvest interval (PHI) of hexythiozox on beans pods was 10 days after the treatment. Based on the results of this study and the relevant residue regulation, hexythiozox residue levels will be acceptable when applied to beans pods in Egypt.