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Abstract
Background . Peritoneal carcinomatosis (PC) is one of the most unfavorable factors of ovarian cancer progression. It is often accompanied by accumulation of fluid in the abdominal cavity, which is called ascites. However, prognostic factors associated with malignant ascites are not well understood. The aim of this study was to evaluate dissimilar populations of EpCAM-positive cells in ascitic fluid and their relationship with the presence of invasive peritoneal implants and the prevalence of carcinomatosis on the Predictive Index Value (PIV) scale in ovarian cancer patients. Materials and methods . The prospective study included 22 patients aged 36–76 years with newly diagnosed FIGO stage Ic–IV ovarian cancer, who were admitted for treatment to Cancer Research Institute of Tomsk NRMC. The study material included EDTA-stabilized ascitic fluid sampled during laparoscopy. Various populations of ascites tumor cells were identified by laser multicolor flow cytometry. The degree of carcinomatosis was determined according to the PIV scale. Results. The study identified twelve populations of EpCAM-positive cells in the ascitic fluid of ovarian cancer patients. Epcam+CD45-CD44-CD24+CD133-Ncadherin+ cells ( r = 0.58; p = 0.004) and atypical (hybrid) EpCam+CD45+CD44+CD24+/-CD133+/-Ncadherin+/- cells ( r = = 0.51; р = 0.01) had a positive correlation with the PIV index. Conclusion . The obtained results show a high degree of heterogeneity of tumor cells in the ascitic fluid of ovarian cancer patients. Identified atypical (hybrid) forms of EpCam-positive cells are of great interest for cell biology and require further investigation.
Highlights
Peritoneal carcinomatosis (PC) is one of the most unfavorable factors of ovarian cancer progression. It is often accompanied by accumulation of fluid in the abdominal cavity, which is called ascites
Prognostic factors associated with malignant ascites are not well understood
The degree of carcinomatosis was determined according to the Predictive Index Value (PIV) scale
Summary
Различные популяции EpСam-положительных клеток в асцитической жидкости у больных раком яичников: связь со степенью канцероматоза. Оценка различных популяций EpСam-положительных опухолевых клеток в асцитической жидкости, их связь с наличием перитонеальных «инвазивных» имплантатов и степенью распространенности канцероматоза по шкале Predictive Index Value (PIV) у больных раком яичников. В проспективное исследование включены 22 больных с впервые диагностированным раком яичников, с Ic–IV стадиями по системе FIGO, в возрасте 36–76 лет, поступившие на лечение в НИИ онкологии Томского НИМЦ. Что клеточный состав асцитической жидкости больных раком яичников представляет собой гетерогенную популяцию. Полученные результаты показывают большую гетерогенность опухолевых клеток в асцитической жидкости больных раком яичников. Ключевые слова: гетерогенность опухолевых клеток, канцероматоз, Predictive Index Value, асцитическая жидкость, рак яичников, жидкостная биопсия. Оригинальные статьи клеток в асцитической жидкости у больных раком яичников: связь со степенью канцероматоза. Kaigorodova E.V.1, 2, Ochirov M.O.1, Molchanov S.V.1, Rogachev R.R.2, Dyakov D.A.2, Chernyshova A.L.1, Shpileva O.V.1, Kovalev O.I.2, Vtorushin S.V.1, 2
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