Disseminated adenovirus infection following allogeneic haemopoetic stem cell transplantation – A report on the use of brincidofovir and 10 year retrospective review of an adult transplant cohort

Abstract

No: 1752 Presentation at ESCV 2015: Oral 38 Salivirus Type 1 and Type 2 in patients with acute gastroenteritis, Germany S. Aldabbagh1,∗, I. Eckerle1, A. Muller2, E.L. Delwart3, A.M. Eis-Hubinger1 1 Institute of Virology, University of Bonn Medical Centre, Germany 2 Department of Neonatology and Pediatric Intensive Care, Childrens Hospital, University of Bonn Medical Centre, Germany 3 Blood Systems Research Institute, Department of Laboratory Medicine, University of California, USA Background: Salivirus (SaV-A) is a novel member of the family Picornaviridae and the only species within the novel picornaviral genus Salivirus. The virus was found in up to 9% of fecal samples from patients, mostly children, with gastroenteritis, with highest prevalences in Asian countries. Thus, the virus was suspected to cause acute gastroenteritis. Recently, a second type of SaV-A (SaVA2) was identified in a sewage sample from Thailand. Because no information is available on SaV-A infection in Western Europe, we analysed stool samples from patients with symptoms suspicious of acute viral gastroenteritis for SaV-A and evaluated the clinical course of SaV-A-positive individuals. Methods: A total of 3019 fecal samples from 1941 hospitalised patients with symptoms of acute gastroenteritis were analysed. Samples were collected during 2012–2013 at the University Hospital Bonn and were routinely screened by (RT-)PCR for human norovirus, sapovirus, astrovirus, rotavirus, and adenovirus. A SaVA real-time RT-PCR was designed targeting a conserved nucleotide sequence in the 5′-untranslated region. Positive results were verified by sequencing the viral capsid protein 1 gene allowing also typing of the virus andwhole genome sequencing. Medical records of SaV-A-infected patients were reviewed for clinical features and laboratory data. Results: SaV-A was detected in five unrelated patients, with viral concentrations ranging between 7.1×106 and 7.2×108 copies/g feces, in the absence of common gastroenteritis viruses. The viruses from four patients were classified as SaV-A1 while in one patient SaV-A2 was present. In none of the five patients SaV-A infection was considered as the major cause of the gastroenteritis symptoms. Conclusion: SaV-A infection can be detected in Germany,Western Europe, albeit at low levels. The detection of SaV-A2 in Europe suggests global spread of SaV-A2. Presence of SaV-A, even at high concentration, in a stool sample provides no conclusive evidence that SaV is the major cause of the patient’s gastroenteritis symptoms. http://dx.doi.org/10.1016/j.jcv.2015.07.048 Abstract No: 1526 Presentation at ESCV 2015: Oral 39 Disseminated adenovirus infection following allogeneic haemopoetic stem cell transplantation – A report on the use of brincidofovir and 10 year retrospective review of an adult transplant cohort I. Ramsay1,∗, C. Attwood2, D. Irish1, D. Lowe3, P.D. Griffiths1, Kyriacou Charalampia2 1 Department of Virology, Royal Free Hospital, London UK 2 Department of Haematology, Royal Free Hospital, London UK 3 Department of Immunology, Royal Free Hospital,