Dissecting the Role of Juvenile Hormone Binding Protein in Response to Hormone and Starvation in the Cotton Bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae).

Abstract

Juvenile hormone (JH) regulates many physiological processes in insect development, diapause, and reproduction. Juvenile hormone binding protein (JHBP), the carrier partner protein of JH, is essential for the balance of JH titer to regulate the metamorphosis and development of insect. In this study, two JHBP genes were identified from Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), namely HaJHBP1 and HaJHBP2. The tissue and temporal expression pattern revealed that both HaJHBP1 and HaJHBP2 were dominantly expressed in larval fat body, and their high transcription stages were detected in fourth and fifth instars. The ingestion of methoprene, a JH analogue, significantly induced the expression of HaJHBP1 and HaJHBP2. However, both HaJHBP1 and HaJHBP2 mRNA levels were significantly downregulated after treated with a JH antagonist, precocene. When subject to starvation, larvae showed a marked suppressive effect in the expression of HaJHBP1 and HaJHBP2. These results indicate that JHBP plays a part in the JH-regulated metabolism, growth, or development in reaction to different nutritional conditions.