Abstract
BackgroundGrapevine is a crop of major economic importance, yet little is known about the regulation of shoot development in grapevine or other perennial fruits crops. Here we combine genetic and genomic tools to identify candidate genes regulating shoot development in Vitis spp.ResultsAn F2 population from an interspecific cross between V. vinifera and V. riparia was phenotyped for shoot development traits, and three Quantitative Trait Loci (QTLs) were identified on linkage groups (LGs) 7, 14 and 18. Around 17% of the individuals exhibited a dwarfed phenotype. A transcriptomic study identified four candidate genes that were not expressed in dwarfed individuals and located within the confidence interval of the QTL on LG7. A deletion of 84,482 bp was identified in the genome of dwarfed plants, which included these four not expressed genes. One of these genes was VviCURLY LEAF (VviCLF), an orthologue of CLF, a regulator of shoot development in Arabidopsis thaliana.ConclusionsThe phenotype of the dwarfed grapevine plants was similar to that of clf mutants of A. thaliana and orthologues of the known targets of CLF in A. thaliana were differentially expressed in the dwarfed plants. This suggests that CLF, a major developmental regulator in A. thaliana, also controls shoot development in grapevine.
Highlights
Grapevine is a crop of major economic importance, yet little is known about the regulation of shoot development in grapevine or other perennial fruits crops
Genetic linkage map construction For linkage mapping, a total of 173 simple sequence repeat (SSR) markers were tested with DNA samples from CS, V. riparia cv
Three loci explain 62.5% of the total variance in internode length (IL) Using an interspecific V. vinifera x V. riparia F2 population with 17% of dwarfed and abnormal individuals, we identified three loci related to IL on LG7, 14 and 18, explaining 33.0, 14.8 and 12.2% of the phenotypic variance, respectively
Summary
Grapevine is a crop of major economic importance, yet little is known about the regulation of shoot development in grapevine or other perennial fruits crops. Grapevine (Vitis spp.) is one of the most economically important perennial fruit crops cultivated worldwide and the regulation of shoot development is central to fruit yield and berry oenological potential. Grown grapevines produce annual shoots that emerge from a given number of latent buds retained after pruning and shoot development results from the recurrent production and development of phytomers. In grapevine (V. vinifera L.), only a small number of shoot development mutants have been identified in grapevine. Other dwarf grapevines have been identified based on gibberellin insensitivity [4, 5]. These dwarf grapevines are of particular interest for genetic studies in small controlled environments [4]
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