Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Masahiro Suzuki,Chihiro Norizuki,Jun-Ichi Wachino,Kumiko Kawamura,Noriyuki Nagano,Yukiko Nagano,Wataru Hayashi,Kouji Kimura,Yohei Doi,Yoshichika Arakawa

doi:10.1016/j.jiac.2021.12.003

Masahiro Suzuki, Chihiro Norizuki + Show 8 more

Open Access

https://doi.org/10.1016/j.jiac.2021.12.003

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Abstract

ObjectivesWe aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. MethodsPhylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). ResultsThe majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. ConclusionOSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.

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