Abstract
Cell suspension of Nannochloropsis oculata was cultured in a modified f/2 medium to study the changes of lipid content during phases of growth. The growth of cell suspension was determined by measuring the cell density and diameter under light microscope. To observe and evaluate the accumulation of lipid droplets in microalgae cells, lipid droplets were stained with Nile Red fluorescent dye then examined under fluorescence microscope and the obtained images were analyzed using Fiji ImageJ, an image processing program. The cell density increased quickly at the first 6 days of culture while cell diameter reached the highest value at the 8th day and 20th day of culture. The presence of lipid droplets in the cells could be observed from the 20th day of culture. The size of lipid droplets was gradually increased after 60 days. Treatment of depleted nitrogen for 4 days resulted an increase in the accumulation of lipid. The intracellular lipid accumulation during phases of growth of the cell suspension under nitrogen-depleted conditions was also discussed.
Highlights
Storage lipids in microalgae is a potential source of biodiesel, an alternative energy replaces fossil fuels [1,2,3]
Fluorescence of lipid droplets staining by Nile Red
The number of fluorescent regions corresponded to the number of lipid droplets in the cells
Summary
Storage lipids in microalgae is a potential source of biodiesel, an alternative energy replaces fossil fuels [1,2,3]. Fluorescence microscope, Nile Red dye, and ImageJ software were used to observe and evaluate the shape, size, and density of some intracellular pigments or lipid droplets in microalgae [4,5,6,7,8,9,10]. The experimental results showed that the lipid yield was enhanced in nitrogen-depleted condition in comparison to Received: 12-9-2017, accepted 20-01-2018, published 2011-2018. Trinh Cam Tu, Tran Thanh Huong, Bui Trang Viet – University of Science, VNU-HCM
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