Abstract
A simple press is described for disrupting solid tissue for preparing plasma membrane. It can handle large amounts of tissue (about 20 g wet weight) per stroke, is readily applicable to the disruption of lymph node, spleen, and placenta, and can be adapted to the breakage of soft nonfibrous tissues such as brain. Plasma membrane was isolated from the disrupted tissue by differential and discontinuous sucrose density gradient centrifugation in yields of between 15 and 25% (relative to theoretical yield). The purified plasma membrane was not contaminated with significant amounts of other subcellular fractions.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
