Abstract
The kinetics of disruption of Rhizopus nigricans ATCC 6227b in a high-pressure homogenizer exhibit major differences from those of unicellular microorganisms. Release of soluble protein is a weak function of pressure and number of passes. The maximum level of soluble protein release, which appeared to vary with pressure, was shown to be the result of micronization of cell debris. The more specific assay possible for a soluble enzyme showed no such apparent variation. Blockage of the homogenizer did not occur for concentrations up to 22 gl −1 (dry weight of cells) of filamentous suspensions, and higher concentration of 30 gl −1 (dry weight of cells) of pelletted cells.
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