Abstract
Over the last years, optical biosensors based on plasmonic nanomaterials have gained great scientific interest due to their unquestionable advantages compared to other biosensing technologies. They can achieve sensitive, direct, and label-free analysis with exceptional potential for multiplexing and miniaturization. Recently, it has been demonstrated the potential of using optical discs as high throughput nanotemplates for the development of plasmonic biosensors in a cost-effective way. This work is a pilot study focused on the development of an integrated plasmonic biosensor for the monitoring of cell adhesion and growth of human retinal pigmented cell line (ARPE-19) under different media conditions (0 and 2% of FBS). We observed an increase of the plasmonic band displacement under 2% FBS compared to 0% conditions over time (1, 3, and 5 h). These preliminary results show that the proposed plasmonic biosensing approach is a direct, non-destructive, and real-time tool that could be employed in the study of living cells behavior and culture conditions. Furthermore, this setup could assess the viability of the cells and their growth over time with low variability between the technical replicates improving the experimental replicability.
Highlights
Monitoring cell adhesion and growth is of crucial importance for a wide range of applications involved in drug screening, cytotoxicity, and cytocompatibility studies
Plasmonic nanocrystals were fabricated using gold-capped polycarbonate templates obtained from commercial Blu-ray optical discs without the need of adhesion layers that can diminish the plasmon-exciton coupling by increased Ohmic plasmon losses (López-Muñoz et al, 2018)
The scanning electron microscopy (SEM) image in Figure 2A demonstrate the presence of high-ordered 70 nm gold-capped nanostructures
Summary
Monitoring cell adhesion and growth is of crucial importance for a wide range of applications involved in drug screening, cytotoxicity, and cytocompatibility studies. Between the different techniques to evaluate or assess cell adhesion, fluorescent and confocal microscopy are the gold standards (Braut-Boucher et al, 1995; Drey et al, 2013; Gomes et al, 2018). These techniques usually require cell labelling that can potentially interfere with the properties of the membrane (Robson et al, 2018). They involve post-detection analysis, which decreases the potential for real-time monitoring of the kinetic of the adhesion process
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