Abstract
Clots obtained from normal native platelet-poor plasma are dispersed by addition of 5 M urea within 120 hours. Clots from plasma diluted > : 4 (Ann. NY. Acad. Sei. 202, 341–343, 1972) and clots from undiluted plasma, thoroughly washed and incubated in veronal buffer pH 7.4, are not dispersed by urea, but they are rendered susceptible to the dispersing action of urea by prior incubation in normal undiluted plasma or serum. The same phenomenon was observed by incubating the otherwise unsoluble clots in normal plasma or serum fractions precipitated at 33% saturation with (NH4)2SO4. The plasma fraction is more active than the serum one. This fraction was tested by casein-14C putrescine Factor XIII assay: it does not interfere with the incorporation of putrescine into casein, although it conditions the urea- dispersing action on stable clots.
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