Dispersal beyond geographic barriers: a contribution to the phylogeny and demographic history of Pristurus rupestris Blanford, 1874 (Squamata: Sphaerodactylidae) from southern Iran

Abstract

Pristurus rupestris is a member of Semaphore geckos with a wide distribution range. Recently, 14 candidate species of P. rupestris rupestris have been identified in the Hajar Mountains (Arabia), yet the knowledge on the Iranian counterparts is limited. The present study elucidates the phylogenetic position of the Iranian P. rupestris and investigates the hypothesis on its historical colonization from Oman to Iran and the associated islands. Therefore, 20 Iranian specimens along with 115 individuals from Oman were examined using two mitochondrial genes including the Cytochrome b and the 12S ribosomal RNA. The molecular phylogenetic analyses revealed that the individuals collected from Iran are well nested within the candidate Species 3 of P. r. rupestris, demonstrating a single population with high gene flow. Additionally, the molecular analyses showed that the genetic diversity within the Iranian Blanford's Semaphore geckos is low and that the candidate Species 3 experienced a recent expansion approximately 17 thousand years ago (Kya). The historical demographic analyses (BSP) showed a mild increase in the effective population size between 15–20 Kya. These time estimations coincide with the Last Glacial Maximum, when the Persian Gulf was almost dry, reinforcing the hypothesis that the species might have colonized southern Iran from Oman through the Persian Gulf. In addition, we propose P. r. iranicus to be synonymized with P. r. rupestris.