Disomic chromosome addition from Thinopyrum intermedium to bread wheat appears to confer stripe rust resistance

Linman Nie,Tihua Fu,Jun Zhang,Yuening Yang

doi:10.1007/s10681-019-2376-7

Abstract

A wheat line 14-569 developed from a hybrid of common wheat cv. Chuannong 10 with the wheat–Thinopyrum intermedium partial amphiploid TAI7045 exhibits stable double spikelets per node character in the lower middle portion of a spike, is highly resistant to stripe rust, and produces kernels with a higher 1000-kernel weight than those of its wheat parent. Chromosome counting and an analysis of meiosis demonstrated that line 14-569 is a disomic addition line with 2n = 44, 22II. Sequential genomic in situ hybridization and non-denaturing fluorescence in situ hybridization analyses indicated that one pair of St-genomic chromosomes from Th. intermedium was added to the wheat complement and that the wheat complement included one pair of 1RS/1BL wheat–rye translocation chromosomes. PCR-based landmark unique gene molecular marker analysis revealed that the added pair of chromosomes comprised 3St chromosomes, which suggested that the added 3St chromosomes from Th. intermedium might carry the genes to control the high yield-related characters and stripe rust resistance. This addition line will potentially be useful resource for improvements in wheat yields and stripe rust resistance.

Highlights

Wheat stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Eriks., is one of the most damaging diseases of wheat and seriously reduces the yield and quality of wheat worldwide (Roelfs et al 1992)
It is important to determine the genetic constitution of alien addition lines in order to produce translocations that compensate for the transfer of genes from alien chromosomes into wheat genomes
We have identified one pair of chromosomes from Th. intermedium in wheat line 14-569 which exhibits a high level of resistance to stripe rust, which is currently a major global problem

Summary

Introduction

Wheat stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Eriks., is one of the most damaging diseases of wheat and seriously reduces the yield and quality of wheat worldwide (Roelfs et al 1992). Yield losses caused by stripe rust can reach 75–100% in the most severe epidemic years under weather conditions that favor the pathogen (Chen 2005b). The most economical, effective and environment-friendly approach for controlling this disease comprises the cultivation of resistant cultivars (Line and Chen 1995). Rapid changes in the virulence of pathogen populations overcome the resistance genes in released wheat cultivars. New resistance resources for stripe rust are continuously required to breed new resistant wheat cultivars. The relatives of wheat possess plenty of desirable genes, including disease-resistance and yield-related genes, for wheat breeding, and these genes can be introduced into wheat by wide crosses and genetic manipulation (Jiang et al 1993)

Methods

Results

Conclusion