Disinfection of caries-affected dentin using activated vitamin B2 and diode laser with total-etch technique. An assessment of adhesive bond

Abstract

AimThe present study aims to evaluate the bond integrity of composite restoration bonded to CAD after disinfection using pineapple peel extract (PPE) Diode laser (DL) and riboflavin (RF) activated by photodynamic therapy (PDT). Material and MethodsForty human permanent mandibular molars were collected, disinfected, and mounted in poly-vinyl pipes. Carious infected dentin was removed and CAD was disinfected. Samples were divided into 4 groups based on disinfection. Group 1: CAD disinfected with CHX; group 2: CAD disinfected with diode laser; CAD disinfected with PPE and CAD sterilized with riboflavin. Following disinfection, CAD surfaces were applied with the etch-and-rinse adhesive system and bonding agent and restored with a composite restoration. Samples were placed in a thermocycler to simulate oral aging and then mounted on the universal testing machine (UTM) for shear bond strength (SBS) testing. Failure mode was assessed using a steriomicroscoscope at 40X magnification. One-way analysis of variance (ANOVA) compared the mean SBS and failure type obtained to identify intergroup differences. Similarly, for multiple group comparison, the Tukey HSD test was used keeping the level of significance at 0.05. ResultsCAD disinfected with chlorhexidine (CHX) established the highest bond integrity of the restoration. However, group 2 CAD disinfected with DL displayed the lowest bond values. It was also observed that dentin disinfected using CHX demonstrated comparable SBS values to CAD disinfected with PPE and CAD disinfected with RF. ConclusionRF and PPE displayed comparable outcomes of SBS of composite restoration bonded to CAD to that of CHX. DL displayed lower bond integrity than the other investigated groups. Further studies are recommended to deduce the findings of the existing study.