Disinfection efficacy and fracture strength of PMMA denture-based polymer with chlorhexidine, PDT utilizing Rose Bengal and hematoporphyrin, and Er, Cr: YSGG laser

Abstract

Objective: The study aimed to assess and compare disinfection efficacy and fracture strength of PMMA based DBPs using chlorhexidine (CHX), PDT utilizing Rose Bengal (RB) and hematoporphyrin HPD, and Er,Cr:YSGG laser (ECL) induced antimicrobial action against in-vitro biofilms colonized with C.albicans, S.aureus, S. mutans, and E. coli.Material and Methods: American Type Culture Collection (ATCC) of C.albicans, S.aureus, S. mutans and E. coli were cultured. Forty-eight PMMA-based denture base plates (DBPs) were prepared by heat-cure acrylic resin and contaminated by in-vitro biofilm under-stimulated in-vitro conditions. DBPs were treated with group1; ECL group 2; RB 5µm, group 3; HPD 500 mg/L and group 4; 0.12% CHX (controls) respectively, for the disinfection of biofilms. All photosensitizers (PS) were activated by LED at a different wavelength. Each contaminated DBP was sprayed on all its surfaces with the aforementioned photosensitizers and CHX. One-way analysis of variance (ANOVA) was used to test the efficacy of disinfection and fracture load testing. Tukey multiple comparison tests were performed to compare means of CFU/mL (log10) for exposed E. coli, C. albicans, S aureus, and S. mutans.Results: Specimens in group 1 disinfected with erbium laser, group 3 disinfected with HPD, and group 4 sterilized with chemical disinfection were effective in decreasing bacterial load CFU/mL (log10) against C.albicans, S. aureus, S.mutans, and E. Coli (p>0.05). Intergroup comparison demonstrated ECL, CHX and HPD demonstrated a comparable reduction against C.albicans and S.aureus (p>0.05). Similarly, S.mutans and E.coli were sensitive against all experimental groups (p>0.05). no significant difference in fractural load analysis among the different investigated groups was noted (p>0.05).Conclusion: ECL and HPD photosensitizer revealed a significant reduction in CFU/ml of exposed viable colonies of C.albicans, S.aureus, S. mutans, and E. coli comparable to 0.12% CHX mediated disinfection of PMMA-based DBPs. Irrespective of the type of disinfection no influence of disinfection was noted on the fracture load of PMMA DBPs.