Abstract

Main target of this work is to explore/delineate the bactericidal properties of natural product mixtures as disinfectants of plant origin. For this purpose, the disinfectant activity of essential oil [1], decoction and hydrosol of Satureja thymbra were tested against bacterial biofilms comparatively with the action of various known chemical decontaminating solutions. Thus, the activities of seven decontaminating solutions were determined against the following five bacterial species (grown either as monospecies or as mixed culture biofilms): Staphylococcus simulans and Lactobacillus fermentum (useful bacteria), Pseudomonas putida (spoilage bacterium), Salmonella enterica and Listeria monocytogenes (pathogenic bacteria). The quantification of their antimicrobial actions was performed using the bead vortexing method, followed by agar plating and/or by conductance measurements (via the metabolic activity of biofilm cells).

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