Disinfectant Properties of Acid Mine Drainage: Its Effects on Enteric Bacteria in a Sewage-Contaminated Stream

Abstract

Abstract Studies conducted in a Cambria County, Pennsylvania, acid mine drainage stream suggest that mine drainage rapidly reduces in situ populations of fecal bacteria associated with inputs of untreated sewage. The density of lactose-fermenting bacteria, mostly coliform species from sewage, declined 1000-fold over a distance of less than 100 m following the input of high acid (pH 3.5 to 4.0), high ferrous iron (45 mg/1) acid mine drainage. Enterobacteriaceae were isolated from the stream, identified, and tested for tolerance to acid mine drainage by exposing cells to drainage for 10 minutes at 0 or 37 °C. Populations of all tested isolates were reduced by this treatment, but some isolates were significantly less affected than others. Thus, while mine drainage may act as a disinfectant, it may not reduce all populations of disease-causing intestinal bacteria at an equal, rapid rate. Population reduction was also temperature- and plating medium-dependent.