Dishevelled family proteins (DVL1-3) expression in intrauterine growth restriction (IUGR) placentas.

Ida Marija Sola,Semir Vranic,Frane Paic,Alan Serman,Paula Slatina,Anita Skrtic,Luka Kakarigi,Valentina Karin-Kujundzic,Ljiljana Serman

doi:10.17305/bjbms.2020.5422

Abstract

Dishevelled family proteins (DVL1, DVL2, and DVL3) are cytoplasmic proteins that are involved in canonical and non-canonical Wnt signaling pathway during embryonic development. The role of DVL proteins in the placental tissue remains mostly unknown. In the current study, we explored the role of Dishevelled proteins in naturally invasive tissue, trophoblast. Formalin-fixed paraffin-embedded samples of 15 term placentas from physiologic term pregnancies and 15 term placentas from pregnancies complicated with intrauterine growth restrictions (IUGR) were used for the study. Expression levels of mRNA for DVL1, DVL2, and DVL3 in placentas were analyzed by quantitative real-time PCR (qRT-PCR). DVL1, DVL2, and DVL3 protein expression were semi-quantitatively analyzed using immunohistochemistry. The expression of DVL3 protein was significantly higher in trophoblasts and endothelial cells in placental villi from IUGR pregnancies compared with the control group of term placentas, while DVL2 protein expression was significantly higher in trophoblasts in placental villi from IUGR pregnancies compared with normal term placentas. The observed differences at protein levels between normal and IUGR placentas were not confirmed at the mRNA levels of DVL genes. Our data indicate the active involvement of DVL proteins in IUGR-related placentas. No significant changes were observed in DVL mRNA levels between the two groups of placentas. Further studies are required to explore the clinical relevance of these observations.

Highlights

The invasion into the endometrium and remodeling of the mother’s spiral arteries are essential steps for the proper functions of the placenta during pregnancy [1]
In intrauterine growth restriction (IUGR) placentas, DVL2 and DVL3 were expressed in >10% of trophoblast cells in 100% and 66.7% of samples, respectively, while in normal placentas, DVL2 and DVL3 were expressed in
Expression of DVL2 and DVL3 was significantly lower in trophoblasts in placental villi from uncomplicated pregnancies (Figure 1B and C) than in trophoblasts in placental villi from IUGR pregnancies (p = 0.016 and p = 0.030, respectively) (Figure 1E and F)

Summary

Introduction

The invasion into the endometrium and remodeling of the mother’s spiral arteries are essential steps for the proper functions of the placenta during pregnancy [1].

Objectives

Methods

Results

Conclusion