Abstract
Thin layer chromatography (TLC) is an analytical method used for separation and detection of components from a mixture. This process is based on interaction in the form of adsorption, partition, ion exchange, size-inclusion/exclusion and ion exchange between stationary phase such as silica gel, modified silica gels, alumina, cellulose, etc. and mobile phase. Various types and characteristics of stationary phase employed in TLC have been discussed in detail in the chapter. There are different techniques and development chamber available for chromatogram development such as ascending, horizontal, 2D, radial and circular which have been described in the chapter. Some common solvent systems used for phytochemical analysis of different groups of herbal drugs have been discussed. Various methods of detection and evaluation of TLC plates such as UV, a detection reagent, quantitative procedures have been described in detail. A separate section is provided for specific procedure such as TLC bioautography, QTLC, application of TLC for assay of steroids and OPTLC. TLC method employed for estimation of various phytochemical groups such as alkaloids, flavonoids, coumarins, and saponins has been discussed. A section demonstrating the practical application of TLC for herbal drug research in the form of case studies has been provided with suitable example in each case.
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