Abstract
Publisher Summary In all vertebrate species, there is 5-HT in the brain, especially in those centers functionally connected with the autonomic system, the reticular activating system or both, suggests a physiologic role in the brain. The fraction that contains synaptosomes provides a suitable starting material for the isolation and identification of unknown transmitter substances in the brain. The presence of bioactive material in this fraction does not prove that it is involved in chemical transmission, but localization suggests its possible implication in transmitter activity. The percentage distribution for 5-HT in the bound-particulate (P) and free-soluble (S) fractions as 68 (60–75) and 32 (25–40) % respectively, found the particulate-to-soluble partition to be 74 and 26% respectively. Brain 5-HT has a distribution similar to that of acetylcholine. Of the three primary fractions, the crude mitochondrial fraction, prepared by differential centrifugation, is the richest in 5-HT. The differential separation between an intermediate speed crude mitochondrial and high speed microsomal fraction is arbitrary. In order to sediment most of the synaptosomes, it is necessary to precipitate the heterogeneous mitochondrial fraction at integrated centrifugal fields of the order of 106 g minute (17,000 g x 1 hour).
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