Abstract
BackgroundKorean native chicken (KNC) is a well-known breed due to its superior meat taste. This breed, however, owing to a low growth rate, has a high market price. In order to overcome this disadvantage, the National Institute of Animal Science (NIAS) in Korea developed a commercial KNC breed, named Woorimatdag version 2 (WM2), an upgraded version of the Woorimatdag (WM1) breed and the WM2 was created by crossing the KNC with meat type breeds. This study aims to discriminate between WM2 and other chicken breeds using microsatellite (MS) markers.MethodsA total of 302 individuals from eight Korean chicken populations were examined. The genetic diversity and population structure analysis were investigated using Cervus, API-CALC, STRUCTURE, PowerMarker programs.ResultsBased on heterozygosity and polymorphic information content (PIC) values, 30 MS markers were initially selected from 150 markers. The identified average number of alleles (Na), expected heterozygosity, and PIC values for the WM2 samples were 7.17, 0.741, and 0.682, respectively. Additionally, the paternity of individuals was assigned with a success rate of greater than 99% using 12 markers, the best minimum number of markers. The 12 selected markers contained heterozygosity and PIC values above 0.7 and probability of identity values around zero. Using these markers, the determined probability of identity (PI), PIhalf-sibs, and PIsibs values were 3.23E-33, 5.03E-22, and 8.61E-08, respectively.ConclusionsWM2 is well differentiated with respect to other chicken breeds based on estimated genetic distances. The results presented here will contribute to the identification of commercial WM2 chicken in the market.Electronic supplementary materialThe online version of this article (doi:10.1186/s40781-015-0044-6) contains supplementary material, which is available to authorized users.
Highlights
Korean native chicken (KNC) is a well-known breed due to its superior meat taste
Since 1992, a Korean native chicken (KNC) conservation project was launched by the National Institute of Animal Science (NIAS) in an attempt to restore local chicken breeds
Polymorphisms of microsatellite markers A total of 215 alleles were detected from 30 MS markers in Woorimatdag version 2 (WM2), ranging from 5 to 13 alleles with an average of 7.17 alleles per locus
Summary
Korean native chicken (KNC) is a well-known breed due to its superior meat taste. This breed, owing to a low growth rate, has a high market price. This study aims to discriminate between WM2 and other chicken breeds using microsatellite (MS) markers. Since 1992, a Korean native chicken (KNC) conservation project was launched by the National Institute of Animal Science (NIAS) in an attempt to restore local chicken breeds. Consumers tend to pay more for the KNCs because of Traditional methods to identify chicken breeds focused on general appearances such as feather color, shank color, Choi et al Journal of Animal Science and Technology (2015) 57:5 and body type [6]. Korean cattle and pork industries developed discrimination and traceability systems using microsatellite (MS) markers [8,9]
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