Abstract
BackgroundNowadays, Radix Polygoni Multiflori (RPM, Heshouwu in Chinese) from different geographical origins were used in clinic. In order to characterize the chemical profiles of different geographical origins of RPM samples, ultra-high performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) combined with chemometrics (partial least squared discriminant analysis, PLS‑DA) method was applied in the present study.MethodsThe chromatography, chemical composition and MS information of RPM samples from 18 geographical origins were acquired and profiled by UPLC-QTOF/MS. The chemical markers contributing the differentiation of RPM samples were observed and characterized by supervised PLS‑DA method of chemometrics.ResultsThe chemical composition differences of RPM samples derived from 18 different geographical origins were observed. Nine chemical markers were tentatively identified which could be used as specific chemical markers for the differentiation of geographical RPM samples.ConclusionsUPLC-QTOF/MS method coupled with chemometrics analysis has potential to be used for discriminating different geographical TCMs. Results will help to develop strategies for conservation and utilization of RPM samples.
Highlights
Nowadays, Radix Polygoni Multiflori (RPM, Heshouwu in Chinese) from different geographical origins were used in clinic
We found that fingerprinting quality control of RPM from different geographical origins was determined by thin-layer chromatography (TLC) scanning and high-performance liquid chromatography (HPLC) [11, 12]; the quality of various commercial specifications of RPM and its dregs was evaluated by HPLC [13]; the quality control of RPM from different origins was determined by infrared spectrum (IR), inductively coupled plasma-atomic emission spectrometer (ICPAES) and LC–mass spectrometry (MS), etc. [14,15,16]
Data analysis by Progenesis QI Multivariate statistical tools was used to observe all differences among the RPM samples from different geographical origins
Summary
Radix Polygoni Multiflori (RPM, Heshouwu in Chinese) from different geographical origins were used in clinic. Radix Polygoni Multiflori (RPM, Heshouwu in Chinese) is the dried root tuber of Polygonum multiflorum Thunb. The RPM dose in the Chinese Pharmacopoeia (2005 edition) is 6–12 g [8]. According to the safety considerations, the recommended dose of RPM was adjusted to 3–6 g in the 2010 edition of the Chinese Pharmacopoeia [9]. The safety, quality and efficacy of RPM samples may vary greatly because of the different geographical origins. Medicinal herbs in authentic producing areas had the best quality, which can produce the best pharmacological effect. Owing to its many origins, the “trueborn area” of RPM is still being studied. The distinction among RPM samples from different origins is essential for determining the trueborn area of RPM and for selecting good quality RPM to treat diseases
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