Discrimination of isogenic wheat by InSb focal plane array chemical imaging

Abstract

Waxy wheat lines differ from their non-waxy wild-type counterparts in their function, end use, and their amylopectin and amylose contents. In certain uses, including breadmaking, blending of some waxy (low amylose) wheat flour with that of normal (wild) wheat has been used to advantage. This fact has brought about interest in developing new lines that incorporate desirable traits from both genetic sources. Such prospects are the motivation for this analytical work. Chemical imaging is a convenient way to objectively discriminant between normal wild parent and triple null waxy and potentially to recognize the contribution of the gene of one parent incorporated into a new cultivar. Wavelength-dependent images of kernel cross-sections highlight either of the pure types. PLS and select PCA factor images also provide discrimination. Single null cross products are not discriminated. Spectra from individual endosperm pixels are selected from images to examine details of the chemical microstructure.