Abstract
Purified virions from seven isolates of the common strain (PVY O ) and six isolates of the tobacco veinal necrotic strain (PVY N ) of potato virus Y were examined by denaturing carrier-ampholyte isoelectric focusing (CA-IEF). All gave a similar pattern of one major and either two or three minor bands. The pattern of all PVY N isolates was identical and the coat protein was acidic (pI=6.1 in all cases), whereas the PVY O isolates could be divided into three distinct groups, all of which were more alkaline (pI=6.8, 7.2, and 7.4). Isoelectric focusing followed by electroblotting and immunodetection showed the bands to be of viral origin. SDS-PAGE gave a single major band of 29.5 kDa in all cases. Isolation of virions from either potato or tobacco gave the same results
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