Discrimination between bacterial species by ratiometric analysis of their carbohydrate binding profile.

Lucienne Otten,Elizabeth Fullam,Matthew I Gibson

doi:10.1039/c5mb00720h

Lucienne Otten, Elizabeth Fullam + Show 1 more

Open Access

https://doi.org/10.1039/c5mb00720h

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Abstract

Antibiotic resistance is a global health concern meaning there is an urgent need for new treatments and diagnostics. Here glycosylated surfaces are used to profile the binding patterns of a range of Gram-negative, Gram-positive and mycobacteria. This enables the creation of 'barcodes' to enable identification and discrimination between the strains, which could not be achieved by single-point glycan binding and offers a new concept in bacteria detection.

Highlights

Antibiotic resistance is a global health concern meaning there is an urgent need for new treatments and diagnostics
Many bacteria infections require an initial adhesion step, for example, the high mannose-binding adhesin FimH is a crucial virulence factor found in uropathogenic Escherichia coli and among enterobacteriacae.[18,19]
Biofilm based infections are responsible for many chronic bacteria associated conditions such as pneumonia[20] and tuberculosis[21] and the most difficult to culture.[22,23,24]

Summary

Introduction

Antibiotic resistance is a global health concern meaning there is an urgent need for new treatments and diagnostics. Glycosylated surfaces are used to profile the binding patterns of a range of Gram-negative, Gram-positive and mycobacteria.

Results

Conclusion