Discrepant roles of a quorum quenching bacterium (Rhodococcus sp. BH4) in growing dual-species biofilms

Abstract

Quorum quenching (QQ) is a promising alternative method for biofilm control. However, a largely unexplored issue is the mechanism through which QQ bacteria interact with biofilm-forming bacteria. Here, we explore inter-species interactions during biofilm development (using 96-well polystyrene plates in a static incubator) between the QQ bacterium Rhodococcus sp. BH4 and sludge bacteria. Experimental results revealed that strain BH4 provoked both competitive (76%) and cooperative (24%) interactions (P < 0.05) in dual-species biofilms after 24 h of incubation (mature biofilm), implying that signal destruction by strain BH4 was strain-dependent. Besides hike in the biofilm biomass (~21%), amount of extracellular polymeric substances (EPS) (25–30 times) and particle size (3.5 times) in the Serratia sp. JSB1 biofilm were increased by str. BH4. This suggests that strain BH4 may only have quenching effects against certain bacteria, and that such effects are overlooked at the community level. Taken together, present results imply that in a given biofilm community, not all QS-bacteria interact similarly with Rhodococcus sp. BH4, either because QS-bacteria are tolerant of strain BH4 or QS-bacteria have more than one mechanism for biofilm development. Overall, the QQ-strategy alone seems ineffective at controlling biofilm development, although it may be used in combination with other strategies.