Abstract
The quorum sensing (QS) system enables bacteria, such as Pseudomonas fluorescens, which are vital psychrotrophic food spoilage bacteria, to control various physiological processes. Hence, targeting the bacterial QS might be a good method to control food spoilage and improve food quality. In this study, we screened the ZINC natural product database to search for potential QS inhibitors (QSIs) of P. fluorescens P07 using receptor-based pharmacophore model and virtual screening methods. A total of 28 compounds with high docking scores were tested for their anti-QS activities. The results showed that 22 compounds possessed anti-QS activities and the hit rate was very high (78.57%). Among them, guaiacol, citral et al. showed potent anti-QS activities. Moreover, the effects of guaiacol and citral on the QS-regulated phenotypes were determined. The results showed that guaiacol and citral significantly inhibited the swimming motility, production of extracellular enzymes, and siderophores of P. fluorescens P07. The inhibitory mechanism on the bacterial QS system was investigated by molecular docking analysis.
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