Discovery of Oligosaccharide Antigens for Semi‐Synthetic Glycoconjugate Vaccine Leads against Streptococcus suis Serotypes 2, 3, 9 and 14**

Shuo Zhang,Julinton Sianturi,Dacheng Shen,Peter H Seeberger,Patricia Priegue,Mauro Sella

doi:10.1002/anie.202103990

Abstract

Streptococcus suis bacteria are one of the most serious health problems for pigs and an emerging zoonotic agent in humans working in the swine industry. S. suis bacteria express capsular polysaccharides (CPS) a major bacterial virulence factor that define the serotypes. Oligosaccharides resembling the CPS of S. suis serotypes 2, 3, 9, and 14 have been synthesized, glycans related to serotypes 2 and 9 were placed on glycan array surfaces to screen blood from infected pigs. Lead antigens for the development of semi‐synthetic S. suis serotypes 2 and 9 glycoconjugate veterinary vaccines were identified in this way.

Highlights

Streptococcus suis causes bacterial infections in farm pigs globally,[1] but it is a commensal bacterium that commonly inhabits the upper respiratory, digestive and reproductive systems of pigs.[2,3] Virulent strains can infect the bloodstream and eventually result in septic shock and meningitis in pigs, but can cause septicemia and meningitis in humans.[+] These authors contributed to this work. [**] A previous version of this manuscript has been deposited on a preprint server
Synthesis of Oligosaccharides Related to S. suis Serotype 2 capsular polysaccharides (CPS)
Angewandte Chemie International Edition published by Wiley-VCH GmbH Angew

Summary

Introduction

Streptococcus suis causes bacterial infections in farm pigs globally,[1] but it is a commensal bacterium that commonly inhabits the upper respiratory, digestive and reproductive systems of pigs.[2,3] Virulent strains can infect the bloodstream and eventually result in septic shock and meningitis in pigs, but can cause septicemia and meningitis in humans. Angewandte Chemie International Edition published by Wiley-VCH GmbH CPSs are able to trigger an adaptive immune response resulting in the production of specific antibodies rendering polysaccharides attractive targets for antibacterial vaccine development.[5] S. suis serotypes are distinguished based on the chemical composition of the capsules.[6,7] Serotypes 1/2, 2, 3, 7, 9 are most frequently isolated from infected animals and differ in geographical prevalence.

Results and Discussion

Conclusion

Conflict of interest