Abstract
SummaryCultivated peanut (Arachis hypogaea L.) is an important grain legume providing high‐quality cooking oil, rich proteins and other nutrients. Shelling percentage (SP) is the 2nd most important agronomic trait after pod yield and this trait significantly affects the economic value of peanut in the market. Deployment of diagnostic markers through genomics‐assisted breeding (GAB) can accelerate the process of developing improved varieties with enhanced SP. In this context, we deployed the QTL‐seq approach to identify genomic regions and candidate genes controlling SP in a recombinant inbred line population (Yuanza 9102 × Xuzhou 68‐4). Four libraries (two parents and two extreme bulks) were constructed and sequenced, generating 456.89–790.32 million reads and achieving 91.85%–93.18% genome coverage and 14.04–21.37 mean read depth. Comprehensive analysis of two sets of data (Yuanza 9102/two bulks and Xuzhou 68‐4/two bulks) using the QTL‐seq pipeline resulted in discovery of two overlapped genomic regions (2.75 Mb on A09 and 1.1 Mb on B02). Nine candidate genes affected by 10 SNPs with non‐synonymous effects or in UTRs were identified in these regions for SP. Cost‐effective KASP (Kompetitive Allele‐Specific PCR) markers were developed for one SNP from A09 and three SNPs from B02 chromosome. Genotyping of the mapping population with these newly developed KASP markers confirmed the major control and stable expressions of these genomic regions across five environments. The identified candidate genomic regions and genes for SP further provide opportunity for gene cloning and deployment of diagnostic markers in molecular breeding for achieving high SP in improved varieties.
Highlights
The cultivated peanut, Arachis hypogaea L., is an important oilseeds crop grown in >100 countries and is consumed worldwide in form of high-quality cooking oil, edible nut, peanut butter and candy
During post-harvested processing, pods are passed through peanut shelling machines to collect kernels which provide rich nutrients such as high-quality oil, proteins and a variety of healthy bioactive compounds for human (Davis and Dean, 2016)
Significant variations for shelling percentage (SP) were found among peanut varieties, for example ranging from 59.9% to 81.0% in the Chinese core collection (Jiang et al, 2013) and from 45.3% to 73.1% in the 256 accessions evaluated in Mediterranean Basin (Yol et al, 2018), suggesting that there is a great potential in the enhancement of SP through breeding
Summary
The cultivated peanut, Arachis hypogaea L., is an important oilseeds crop grown in >100 countries and is consumed worldwide in form of high-quality cooking oil, edible nut, peanut butter and candy. Peanut is an allotetraploid grain legume (AABB, 2n = 4x = 40) with genome size of ~2.7 Gb (Kochert et al, 1996). It is known as the groundnut due to underground pod development. During post-harvested processing, pods are passed through peanut shelling machines to collect kernels (seeds) which provide rich nutrients such as high-quality oil, proteins and a variety of healthy bioactive compounds for human (Davis and Dean, 2016). Shelling percentage (SP), a ratio in percentage of the weight of kernels to the weight of pods, is an important economic trait in peanut production. Significant variations for SP were found among peanut varieties, for example ranging from 59.9% to 81.0% in the Chinese core collection (Jiang et al, 2013) and from 45.3% to 73.1% in the 256 accessions evaluated in Mediterranean Basin (Yol et al, 2018), suggesting that there is a great potential in the enhancement of SP through breeding
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
