Abstract
In spite of its economic importance, very little molecular genetics and genomic research has been targeted at the family Paulownia spp. The little genetic information on this plant is a big obstacle to studying the mechanisms of its ability to resist Paulownia Witches’ Broom (PaWB) disease. Analysis of the Paulownia transcriptome and its expression profile data are essential to extending the genetic resources on this species, thus will greatly improves our studies on Paulownia. In the current study, we performed the de novo assembly of a transcriptome on P. tomentosa × P. fortunei using the short-read sequencing technology (Illumina). 203,664 unigenes with a mean length of 1,328 bp was obtained. Of these unigenes, 32,976 (30% of all unigenes) containing complete structures were chosen. Eukaryotic clusters of orthologous groups, gene orthology, and the Kyoto Encyclopedia of Genes and Genomes annotations were performed of these unigenes. Genes related to PaWB disease resistance were analyzed in detail. To our knowledge, this is the first study to elucidate the genetic makeup of Paulownia. This transcriptome provides a quick way to understanding Paulownia, increases the number of gene sequences available for further functional genomics studies and provides clues to the identification of potential PaWB disease resistance genes. This study has provided a comprehensive insight into gene expression profiles at different states, which facilitates the study of each gene’s roles in the developmental process and in PaWB disease resistance.
Highlights
The Paulownia plant is a fast-growing deciduous hardwood species that belongs to the monogeneric family Paulowniaceae, which is sometimes included in the family Scrophulariaceae
The 335,278,558 high quality reads with 28,687,625,164 nucleotides, an average length of 85 bp and a Q20 percentage of 95%, were chosen for assembly (Table 2). These short reads were assembled de novo into 203,664 unigenes using the Trinity programs
We provided the first publicly available transcriptome resource for P. tomentosa 6 P. fortunei
Summary
The Paulownia plant is a fast-growing deciduous hardwood species that belongs to the monogeneric family Paulowniaceae, which is sometimes included in the family Scrophulariaceae. This species is native to China, where it has been cultivated for over 2,000 years. The wood of Paulownia is lightweight, strong, soft, straight-grained, and mostly knot-free with a satiny luster [5]. It has a wide variety of commercial uses ranging from log homes, non-structural building uses, and paper making to furniture and musical instrument production [4,6,7]. Besides its extensive use as a forestry tree, Paulownia can be used for pollution control (both air and soil), land reclamation and as a fast-growing ornamental tree providing both shade and very attractive flowers [8,9,10,11]
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