Abstract
A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.
Highlights
Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK
To facilitate the transition from research to industrial scale production of adherent cell types, the ideal culture system should comprise of both defined medium and substrate that can be readily-used with existing cultureware from which a stem cell factory can be constructed12
Xenogenic components in the culture system create a barrier to clinical translation as they face greater regulatory hurdles
Summary
Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK. Multi-generation high throughput polymer incorporating high throughput surface characterization (HT-SC)[16] was used to identify microarray screening methodology materials that can support HUES7 (hESC) cell attachment and pluripotency in the widely used commercial defined, serum- and feeder-free medium, StemPro. The first-generation array, consisting of 141 monomers of wide chemical diversity, was printed using metal pins to transfer the liquid monomers onto poly(2-hydroxyethyl methacrylate) (polyHEMA) coated glass slides containing 6 replicates of each homopolymer (Figure 1a - monomer structures presented in Figure S1 and Table S1).[17] Polymer microarray spots of diameters ranging from
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