Abstract
BackgroundSmall extracellular vesicles (small-EVs) are membranous vesicles that contain unique information regarding the condition of cells and contribute to the recruitment and reprogramming of components associated with the tumor environment. Therefore, many researchers have suggested that small-EV proteins are potential biomarkers for diseases such as cancer. Colon cancer (CC) is one of the most common causes of cancer-related deaths worldwide. Biomarkers such as carcinoembryonic antigen (CEA) show low sensitivity (~ 40%), and thus the demand for novel biomarkers for CC diagnosis is increasing.MethodsIn this study, we identified biomarkers for diagnosing CC through proteomic analysis of small-EVs from CC cell lines. These small-EVs were characterized by western blot analysis, nanoparticle tracking analysis, and transmission electron microscopy and analyzed using mass spectrometry.ResultsFive selected proteins were found to be upregulated in CC by western blot analysis. Among the candidate proteins, tetraspanin 1 (TSPAN1) was found to be upregulated in plasma EVs from CC patients compared to those from healthy controls (HCs) with 75.7% sensitivity.ConclusionsThese results suggest that TSPAN1 is a potent non-invasive biomarker for CC detection. Our experimental strategy provides useful insights into the identification of cancer-specific non-invasive biomarkers.
Highlights
Small extracellular vesicles are membranous vesicles that contain unique information regarding the condition of cells and contribute to the recruitment and reprogramming of components associated with the tumor environment
The results suggest that an optimized enzyme-linked immunosorbent assay (ELISA) method can detect surface proteins in small-Extracellular vesicles (EVs) and that small-EV proteins can effectively distinguish between Healthy control (HC) and cancer patients
Isolation and characterization of small-EVs in colon cancer cell lines To identify potential biomarkers for diagnosing colon cancer, HT-29 and HCT-116 human colon cancer cell lines were used in this study
Summary
Small extracellular vesicles (small-EVs) are membranous vesicles that contain unique information regarding the condition of cells and contribute to the recruitment and reprogramming of components associated with the tumor environment. Blood-based biomarkers can be analyzed quickly, and economically, and have the Extracellular vesicles (EVs) are membrane-bound vesicles secreted by a variety of cell types and are found in various body fluids including the blood, urine, breast milk, and malignant ascites [9]. These vesicles contain oncogenic proteins [9], signaling molecules [10], lipids [11], mRNAs, and microRNAs [12] that reflect parental cell functions [13], and can be horizontally transferred to recipient cells to regulate their characterization [12]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
