Abstract
Lung adenocarcinoma (LUAD) is a significant subtype of lung cancer, and understanding the roles of tumor suppressor genes in LUAD remains crucial. In this study, we aimed to identify and experimentally validate potential tumor suppressor genes selected from online databases. By analyzing the TCGA cancer database, we identified genes exhibiting significant differential expression between normal and tumor tissues in LUAD, particularly focusing on genes with lower expression levels in tumors associated with shortened survival periods. Subsequently, we selected UNC5B, DAB2IP, SEMA3F, PPM1L, and AXIN2 as candidate genes and performed knockdown experiments using LUAD cell line HCC827. Our findings revealed that all selected genes, except UNC5B, conferred tumor-like properties when knocked out, suggesting their potential as anti-tumor agents. These results contribute to the understanding of LUAD pathogenesis and highlight the significance of studying tumor suppressor genes in the context of cancer development and patient survival.
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