Abstract
Discopersicus iranicus n. gen., n. comb., previously described from Iran as a new species under the genus Discotylenchus, is illustrated using light microscope and scanning electron microscope (SEM) observations and further studied using molecular characters. SEM studies revealed the newly proposed genus has oblique amphidial apertures on the lateral sides of the lip region. SEM images are also provided for two species of Discotylenchus, namely D. discretus and D. brevicaudatus, as the first SEM study of the genus. These results confirmed longitudinal amphidial aperture type on lateral sides of the lip region in genus Discotylenchus, as noted by Siddiqi while erecting the genus with D. discretus as the type species. Molecular phylogenetic analyses using partial small subunit (SSU) and large subunit (LSU) rDNA sequences revealed the affinity of the genus Discopersicus n. gen. with members of the subfamily Boleodorinae, as supported by morphological characters (mainly, the oblique amphidial opening).
Highlights
Discopersicus iranicus n. gen., n. comb., previously described from Iran as a new species under the genus Discotylenchus, is illustrated using light microscope and scanning electron microscope (SEM) observations and further studied using molecular characters
During our studies on tylenchids in southwestern Iran, a tylenchid nematode population with a perioral disc was recovered, and after detailed study, it was assigned to the species formerly described as Discotylenchus iranicus Ghaemi, Pourjam, Atighi, Pedram and Karssen, 2012
Additional morphological and molecular phylogenetic studies were performed on the recovered population, and based on the newly obtained data, it was revealed that the population belongs to a new genus, belonging to the subfamily Boleodorinae, named in this present study as Discopersicus n. gen., currently a monotypic genus with D. iranicus (Ghaemi et al, 2012) n. comb. as the type species
Summary
Several soil samples were collected from southwestern Iran. To obtain a cleaner suspension of nematodes, the tray method (Whitehead and Hemming, 1965) was employed for extraction. Nematodes of interest were handpicked under a Nikon SMZ1000 stereomicroscope (Nikon Corp., Tokyo, Japan). The collected specimens were killed in hot 4% formaldehyde solution, transferred to anhydrous glycerin according to De Grisse (1969), and mounted in permanent slides. The specimens were examined with a Nikon Eclipse E600 light microscope (Nikon Corp., Tokyo, Japan). The morphometric symbols and used abbreviations here follow Siddiqi (2000). Specimens of the two species of Discotylenchus used for SEM studies were obtained from the material reported by Ghaemi et al (2012)
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